Sensitive biologics can destabilize if frozen or heated improperly, resulting in unsafe and unusable final product or service. They're also delicate to various interactions in the lyophilization process which can result in the proteins to unfold, causing loss of therapeutic performance to the patient2. As the volume of biologic molecules while in
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Inside the ionization chamber the remaining molecules—a combination with the cellular section factors and solutes—undergo ionization and fragmentation. The mass spectrometer’s mass analyzer separates the ions by their mass-to-charge ratio (m/z). A detector counts the ions and shows the mass spectrum.Most common HPLC is executed With all the s